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효율적인 Refolding 및 Chemoreceptor Ligand 바인딩 도메인의 정화 방법

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

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효율적인 Refolding 및 Chemoreceptor Ligand 바인딩 도메인의 정화 방법

17,391 Views

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14:25 min

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December 12, 2017

DOI:

10.3791/57092-v

DOI:

10.3791/57092-v

•

14:25 min

December 12, 2017

•

15 Views

Mayra A. Machuca^1,2, Anna Roujeinikova^1,2,3

¹Infection and Immunity Program,Monash Biomedicine Discovery Institute, ²Department of Microbiology,Monash University, ³Department of Biochemistry and Molecular Biology,Monash University

Summary

프로시저 refolding dCACHE periplasmic ligand 바인딩 도메인 포함 시체와 단백질의 밀리 그램 수량을 정화에서 Campylobacter jejuni chemoreceptor Tlp3의의 대 한 제공 됩니다.

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