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שיטת Refolding יעילה וטיהור של ליגנד Chemoreceptor מחייב תחום
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Bioquímica
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JoVE Journal Bioquímica
Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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שיטת Refolding יעילה וטיהור של ליגנד Chemoreceptor מחייב תחום

17,391 Views

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14:25 min

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December 12, 2017

DOI:

10.3791/57092-v

DOI:
10.3791/57092-v

•

14:25 min
December 12, 2017

•

15 Views
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1Infection and Immunity Program,Monash Biomedicine Discovery Institute, 2Department of Microbiology,Monash University, 3Department of Biochemistry and Molecular Biology,Monash University

Summary

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הליך זה הציג את refolding של התחום מחייב ליגנד periplasmic dCACHE של קמפילובקטר jejuni chemoreceptor Tlp3 מן ההכללה וגופי הטיהור להניב כמויות מיליגרם של חלבון.

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Cite this Article

Machuca, M. A., Roujeinikova, A. Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain. J. Vis. Exp. (130), e57092, doi:10.3791/57092 (2017).

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