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Inmunoprecipitación de cromatina (ChIP) de modificaciones de las histonas de Saccharomyces cerevisiae

JoVE Journal
Genética

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JoVE Journal Genética

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

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Inmunoprecipitación de cromatina (ChIP) de modificaciones de las histonas de Saccharomyces cerevisiae

12,411 Views

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11:06 min

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December 29, 2017

DOI:

10.3791/57080-v

DOI:

10.3791/57080-v

•

11:06 min

December 29, 2017

•

4 Views

Meagan Jezek, Alison Jacques, Deepika Jaiswal, Erin M. Green

¹Department of Biological Sciences,University of Maryland

Tags

Chromatin Immunoprecipitation ChIP Histone Modifications Saccharomyces Cerevisiae Gene Expression Control Environmental Conditions Chromatin Binding Proteins Epitope Tags Antibodies Yeast Cells YPD Medium Optical Density Formaldehyde Glycine ChIP Lysis Buffer PMSF Protease Inhibitor Glass Beads Bead-beating

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