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Ex Vivo Infección de la Mucosa Genital tejido linfoide humano y mujer con Virus de inmunodeficiencia humana 1 y Histoculture
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
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Ex Vivo Infección de la Mucosa Genital tejido linfoide humano y mujer con Virus de inmunodeficiencia humana 1 y Histoculture

8,897 Views

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11:14 min

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October 12, 2018

DOI:

10.3791/57013-v

DOI:
10.3791/57013-v

•

11:14 min
October 12, 2018

•

2 Views
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1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital,Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development,National Institutes of Health

Summary

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Infección de tejidos humanos con virus de inmunodeficiencia humana (VIH) ex vivo proporciona un valioso modelo 3D de la patogenesia virus. Aquí, describimos un protocolo e infectar a los especímenes del tejido de las amígdalas humanas y de la mucosa genital femenina con HIV-1 y mantener en la cultura en la interfase líquido-aire.

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Cite this Article

Introini, A., Vanpouille, C., Fitzgerald, W., Broliden, K., Margolis, L. Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture. J. Vis. Exp. (140), e57013, doi:10.3791/57013 (2018).

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