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精製マウス肝類洞内皮細胞と肝細胞の灌流
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
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精製マウス肝類洞内皮細胞と肝細胞の灌流

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08:54 min

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February 12, 2018

DOI:

10.3791/56993-v

DOI:
10.3791/56993-v

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08:54 min
February 12, 2018

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46 Views
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1Department of Biochemistry,University of Nebraska

Summary

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このプロトコルの目的は、肝臓から高生存率および肝細胞と類洞内皮細胞の高収率を取得することです。これは、灌肝門脈、肝細胞と類洞内皮細胞を取得する差動遠心分離の続くタイプ IV コラゲナーゼの解決で。

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Cite this Article

Cabral, F., Miller, C. M., Kudrna, K. M., Hass, B. E., Daubendiek, J. G., Kellar, B. M., Harris, E. N. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (132), e56993, doi:10.3791/56993 (2018).

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