Journal
/
Bioquímica
/
小鼠肝灌注肝细胞和正弦内皮细胞的纯化
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
Please note that all translations are automatically generated. Click here for the English version.

小鼠肝灌注肝细胞和正弦内皮细胞的纯化

48,836 Views

•

08:54 min

•

February 12, 2018

DOI:

10.3791/56993-v

DOI:
10.3791/56993-v

•

08:54 min
February 12, 2018

•

46 Views
, , , , , ,
1Department of Biochemistry,University of Nebraska

Summary

Automatically generated

该协议的目的是获得高生存能力和高产量的肝细胞和正弦内皮细胞。这是通过灌注肝与 IV 型胶原酶溶液通过门静脉, 其次是差异离心获得肝细胞和正弦内皮细胞。

Explore More Videos

Mouse Liver PerfusionHepatocyte PurificationSinusoidal Endothelial Cell PurificationLiver Cell PopulationsPortal Vein CatheterizationLiver DigestionLiver Cell ViabilityHepatocyte HarvestingBuffer Solutions

Read Article

Cite this Article

Cabral, F., Miller, C. M., Kudrna, K. M., Hass, B. E., Daubendiek, J. G., Kellar, B. M., Harris, E. N. Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion. J. Vis. Exp. (132), e56993, doi:10.3791/56993 (2018).

Download .ris file

Copy

Share Video

.

Copy