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Neuron-Macrophage co culturen te activeren van macrofagen afscheidende moleculaire factoren met Neurite uitgroei activiteit
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Neurociência
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JoVE Journal Neurociência
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
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Neuron-Macrophage co culturen te activeren van macrofagen afscheidende moleculaire factoren met Neurite uitgroei activiteit

8,508 Views

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08:52 min

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March 30, 2018

DOI:

10.3791/56920-v

DOI:
10.3791/56920-v

•

08:52 min
March 30, 2018

•

4 Views
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1Department of Brain Science,Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences,Ajou University School of Medicine, 3Department of Neurology,Ajou University School of Medicine

Summary

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Het huidige protocol presenteert experimentele procedures ter stimulering van gekweekte macrofagen te worden begiftigd met capaciteit om moleculaire factoren ter bevordering van de uitgroei van de neurite vrij te geven. Behandeling van kamp tot de neuron-macrofaag co culturen induceert de macrofagen produceren geconditioneerde medium dat sterke neurite uitgroei activiteit bezit.

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Cite this Article

Yun, H. J., Kim, E., Kim, B. G. Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity. J. Vis. Exp. (133), e56920, doi:10.3791/56920 (2018).

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