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JoVE Journal Neuroscience
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
 

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Article DOI: 10.3791/56920-v • 08:52 min • March 30th, 2018
March 30th, 2018 •
1,2, 1,2, 1,2,3
1Department of Brain Science, Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University School of Medicine, 3Department of Neurology, Ajou University School of Medicine

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The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.

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Neuron-Macrophage Co-cultures Macrophages Molecular Factors Neurite Outgrowth Activity Activation Pro-regenerative Macrophagic Phenotype Regeneration Cyclic AMP Adenosine Serum Molecule Axon Growth Capacity Neurons Permanent Lobe Injury Six Well Plate Poly-D Lycine Laminin Solution Incubate Wash Dry Euthanized Mouse
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