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Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
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JoVE Journal Neurowissenschaften
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

DOI:
10.3791/56920-v

•

08:52 min

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March 30, 2018

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, ,
1Department of Brain Science,Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences,Ajou University School of Medicine, 3Department of Neurology,Ajou University School of Medicine

Kapitel

  • 00:04Titel
  • 01:06Culture Preparation of Dissociated Adult DRG Neuron
  • 03:41Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
  • 05:47Neurite Outgrowth Assay with Collected Conditioned Medium
  • 07:05Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
  • 07:55Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.

Tags

Neuron-macrophage Co-cultureMacrophage ActivationNeurite OutgrowthRegenerationDRGCollagenaseCell CultureDMEM

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