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细胞聚集法评价果蝇切口与反式配体的结合及其对顺式配体的抑制作用
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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细胞聚集法评价果蝇切口与反式配体的结合及其对顺式配体的抑制作用

DOI:
10.3791/56919-v

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05:48 min

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January 02, 2018

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,
1Department of Molecular and Human Genetics,Baylor College of Medicine, 2Program in Developmental Biology,Baylor College of Medicine

Capítulos

  • 00:05Título
  • 00:38Assessment of Binding Between Notch Receptor and Trans-ligands
  • 01:47Aggregation Between Signal-sending and Signal-receiving Cells
  • 03:11Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
  • 04:15Results: Faithful Analysis of Notch Signaling
  • 05:30Conclusion

Summary

Tadução automática

Tadução automática

复杂的在体内系统, 使得很难区分的激活和抑制的缺口受体的跨-和cis配体, 分别。在这里, 我们提出了一个基于体外细胞聚合的方法, 用于定性和半定量评价的结合的果蝇缺口, 以跨配基与顺式配基。

Tags

Cell Aggregation AssayNotch ReceptorTrans-ligandsCis-ligandsDrosophilaS2 CellsLigand BindingCell SignalingAggregation QuantificationMicroscopyHemacytometer

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