需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
7,402 Views
•
05:48 min
•
January 02, 2018
DOI:
Summary
Automatically generated
Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.
Read Article
Cite this Article
Pandey, A., Jafar-Nejad, H. Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands. J. Vis. Exp. (131), e56919, doi:10.3791/56919 (2018).
Copy