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比率钙显像对单个神经元的行为线虫线虫
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
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比率钙显像对单个神经元的行为线虫线虫

DOI:
10.3791/56911-v

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11:26 min

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February 07, 2018

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, , , , ,
1Neuroscience Program,University of Miami School of Medicine, 2Department of Biology,University of Miami

Capítulos

  • 00:04Título
  • 01:03Animal Mounting
  • 02:44Imaging Setup
  • 05:36Ratiometric HSN Ca2 Imaging and C. elegans Behavior Recording
  • 07:09Image Segmentation and Quantitative Analysis
  • 08:58Results: Representative HSN Imaging During Egg-laying
  • 10:35Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了使用基因编码的 Ca2记者记录在行为上的神经活动的变化, 在线虫线虫蠕虫。

Tags

Keywords: Ratiometric Calcium ImagingCaenorhabditis ElegansHSN NeuronBehavioral NeuroscienceGCaMP5MCherryNematode Growth MediumOP50 BacteriaVulvaInverted MicroscopeInfrared FilterVideo AnalysisStage XY Stage Control

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