/

/

监测果蝇中朊病毒样蛋白聚集物的细胞间传输

JoVE Journal
Neurociência

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Neurociência

Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster

Please note that all translations are automatically generated. Click here for the English version.

监测果蝇中朊病毒样蛋白聚集物的细胞间传输

DOI:

10.3791/56906-v

•

10:26 min

•

March 12, 2018

•

Kirby M. Donnelly, Margaret M. P. Pearce

¹Department of Biological Sciences,University of the Sciences

Capítulos

00:04Título
00:58Generating Huntingtin-mosaic Transgenic Flies and Adult Brain Dissection
03:39Whole Brain Fixation, Washing, and Mounting
06:11Imaging and Quantifying Prion-like Transmission of Aggregates
08:22Results: Example Images
09:43Conclusion

Summary

Tadução automática

积累的证据支持这种观点, 即与神经退行性疾病相关的致病蛋白聚集物在细胞间扩散, 与朊病毒类似。在这里, 我们描述了一种方法, 使可视化的细胞到细胞传播的朊病毒样聚集在模型有机体,果蝇。

Tags

Prion-like Protein Aggregates Drosophila Melanogaster Cell-to-cell Transmission Neurodegeneration Transgenic Flies Protein Aggregation Huntingtin Brain Dissection PBST Forceps

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

标记的单细胞在中枢神经系统<em>果蝇</em

Optogenetic刺激的逃避行为<em>果蝇</em

在自发活动的测定<em>果蝇</em

简单的检测方法的社交回避的量化<em>果蝇</em

在条件影响社会空间<em>果蝇</em

一个行为分析对机械感觉MARCM为基础的克隆<em>果蝇</em

觅食路径长度协议<em>果蝇</em>幼虫

毛细管馈线分析测量的摄食<em>果蝇</em

测量并改变配套驱动马累<em>果蝇</em

果蝇幼虫和成年脑的代谢分析

Read Article