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Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster
Chapters
- 00:04Title
- 00:58Generating Huntingtin-mosaic Transgenic Flies and Adult Brain Dissection
- 03:39Whole Brain Fixation, Washing, and Mounting
- 06:11Imaging and Quantifying Prion-like Transmission of Aggregates
- 08:22Results: Example Images
- 09:43Conclusion
Accumulating evidence supports the idea that pathogenic protein aggregates associated with neurodegenerative diseases spread between cells with prion-like properties. Here, we describe a method that enables visualization of cell-to-cell spreading of prion-like aggregates in the model organism, Drosophila melanogaster.
