Journal
/
Bioquímica
/
Verwendung von dreifarbigen Einzelmolekül-Bund, die Korrelation von Protein-Interaktionen zu studieren
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Please note that all translations are automatically generated. Click here for the English version.

Verwendung von dreifarbigen Einzelmolekül-Bund, die Korrelation von Protein-Interaktionen zu studieren

9,796 Views

•

11:22 min

•

January 30, 2018

DOI:

10.3791/56896-v

DOI:
10.3791/56896-v

•

11:22 min
January 30, 2018

•

1 Views
, , ,
1Institute of Physical Chemistry,University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft,Delft University of Technology

Tags

Three-color Single-molecule FRETProtein InteractionsCooperativityQuantitative BiologyProtein ComplexesDynamic InteractionsFlow Chamber AssemblyPEG-biotin Functionalized SlideMulti-color Prism TIRF MicroscopeEMCCD Camera Configuration

Read Article

Cite this Article

Götz, M., Wortmann, P., Schmid, S., Hugel, T. Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions. J. Vis. Exp. (131), e56896, doi:10.3791/56896 (2018).

Download .ris file

Copy

Share Video

.

Copy