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Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice
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Neurociência
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JoVE Journal Neurociência
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

DOI:
10.3791/56758-v

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09:05 min

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February 22, 2018

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, , , ,
1URVI, NARILIS,Université de Namur, 2URPhyM, NARILIS,Université de Namur

Capítulos

  • 00:05Título
  • 01:23Preparations
  • 02:22Intrapleural Injections Using Transdiaphragmatic Approach
  • 04:48Intrapleural Injection using Transthoracic Approach and Post-operative Care
  • 06:38Results: Labeling Neurons with CTB after a C4 Spinal Contusion
  • 08:01Conclusion

Summary

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Here, we describe a protocol for identifying phrenic motor neurons in mice after intrapleural delivery of fluorophore conjugated cholera toxin subunit beta. Two techniques are compared to inject the pleural cavity: transdiaphragmatic versus transthoracic approaches.

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Keywords: Retrograde Neuroanatomical TracingPhrenic Motor NeuronsMiceIntrapleural DeliveryFluorophore-conjugated Cholera Toxin Subunit BetaTransdiaphragmatic ApproachTransthoracic ApproachNeuromuscular JunctionsPleural SpaceSterile SurgeryAnesthesiaSkin PreparationCTB SolutionMicrosyringeLaparotomy

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