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抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

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抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

DOI:

10.3791/56689-v

•

10:35 min

•

December 26, 2017

•

Lisa Kozicky, Laura M. Sly

¹British Columbia Children’s Hospital Research Institute,University of British Columbia

Capítulos

00:05Título
00:44Bone Marrow Macrophage Derivation and Activation with Antibodies: In Vitro Testing
05:34Harvesting Peritoneal Macrophages after an IVIg + LPS Challenge: In Vitro Testing
08:38Results: BMDM Reaction to IVIg + LPS Treatment
10:06Conclusion

Summary

Tadução automática

抗体药物对炎症性疾病进行了革命性的治疗。除了对特定靶点产生直接影响外, 抗体还能激活巨噬细胞成为抗炎药。该协议描述了如何抗炎巨噬细胞活化可以评估在体外,使用小鼠骨髓巨噬细胞, 和在体内,使用腹腔巨噬细胞。

Tags

Antibody-based Drugs Murine Bone Marrow Peritoneal Macrophages Anti-inflammatory IL-10 Immunology Primary Macrophages Bone Marrow In Vitro Peritoneal Cavity In Vivo Euthanized Mouse Bone Marrow Isolation Bone Marrow Culture MCSF

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