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Rab10 fosforylering detectie door LRRK2 activiteit met behulp van SDS-pagina met een fosfaat-bindende-Tag

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

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Rab10 fosforylering detectie door LRRK2 activiteit met behulp van SDS-pagina met een fosfaat-bindende-Tag

14,036 Views

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08:55 min

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December 14, 2017

DOI:

10.3791/56688-v

DOI:

10.3791/56688-v

•

08:55 min

December 14, 2017

•

4 Views

Genta Ito, Taisuke Tomita²

¹Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, ²Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Tags

Rab10 Phosphorylation LRRK2 Activity SDS-PAGE Phosphate-binding Tag Parkinson’s Research HEK293 Cells Cell Lysis Bradford Assay Sample Preparation SDS-PAGE Gel Manganese Chloride

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