Journal
/
Bioquímica
/
用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Please note that all translations are automatically generated. Click here for the English version.

用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

14,036 Views

•

08:55 min

•

December 14, 2017

DOI:

10.3791/56688-v

DOI:
10.3791/56688-v

•

08:55 min
December 14, 2017

•

4 Views
,
1Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

Tags

Rab10 PhosphorylationLRRK2 ActivitySDS-PAGEPhosphate-binding TagParkinson’s ResearchHEK293 CellsCell LysisBradford AssaySample PreparationSDS-PAGE GelManganese Chloride

Read Article

Cite this Article

Ito, G., Tomita, T. Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag. J. Vis. Exp. (130), e56688, doi:10.3791/56688 (2017).

Download .ris file

Copy

Share Video

.

Copy