/
/
DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
9,311 Views
•
08:11 min
•
January 28, 2018
DOI:
Summary
Automatically generated
This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.
Read Article
Cite this Article
Paredes, A. J., Alfaro-Valdés, H. M., Wilson, C. A. DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure. J. Vis. Exp. (131), e56528, doi:10.3791/56528 (2018).
Copy