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DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure

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08:11 min

January 28, 2018

DOI:

08:11 min
January 28, 2018

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Summary

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This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.

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