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DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
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DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure
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January 28, 2018
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Summary
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This method allows selective staining and quantification of DNA in gels by soaking the gel in a SYBR Green I/Nitro Blue Tetrazolium solution and then exposing it to sunlight or a blue light source. This produces a visible precipitate and requires almost no equipment, making it ideal for field use.
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Paredes, A. J., Alfaro-Valdés, H. M., Wilson, C. A. DNA Staining Method Based on Formazan Precipitation Induced by Blue Light Exposure. J. Vis. Exp. (131), e56528, doi:10.3791/56528 (2018).
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