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天然铀对体外破影响的分析方法

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

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天然铀对体外破影响的分析方法

DOI:

10.3791/56499-v

•

09:51 min

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January 30, 2018

•

Tatiana Gritsaenko, Valérie Pierrefite-Carle, Gaëlle Creff, Claude Vidaud, Georges Carle, Sabine Santucci-Darmanin

¹UMR E-4320 TIRO-MATOs CEA/DRF/BIAM,Université Nice Sophia-Antipolis, ²UMR 7272 Institut de Chimie de Nice CNRS,Université Nice Sophia-Antipolis, ³CEA, Direction de la Recherche Fondamentale (DRF),Biosciences and Biotechnologies Institute (BIAM)

Capítulos

00:05Título
00:38RAW 264.7 Differentiation in the Presence of Uranyl Ions [U(VI)]
03:14Osteoclast Size and Morphology Analyses
05:27Osteoclast Function in the Presence of U(VI)
08:30Results: Representative Effects of U(VI) on Osteoclast Size and Function
09:34Conclusion

Summary

Tadução automática

众所周知, 铀会影响骨骼的新陈代谢。在这里, 我们提出了一项协议, 目的是研究天然铀暴露对破骨细胞的活性、分化和功能的影响, 这是负责骨质吸收的单元。

Tags

Keywords: Osteoclastogenesis Uranium Exposure RAW 264.7 Cells Cell Culture Cell Seeding Density Uranium Salt Solution RANKL Osteoclast Differentiation Osteoclast Function Environmental Toxicity Bone Physiology Bone Metabolism

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