Journal
/
发育生物学
/
Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

7,995 Views

•

09:51 min

•

January 30, 2018

DOI:

10.3791/56499-v

DOI:
10.3791/56499-v

•

09:51 min
January 30, 2018

•

1 Views
, , , , ,
1UMR E-4320 TIRO-MATOs CEA/DRF/BIAM,Université Nice Sophia-Antipolis, 2UMR 7272 Institut de Chimie de Nice CNRS,Université Nice Sophia-Antipolis, 3CEA, Direction de la Recherche Fondamentale (DRF),Biosciences and Biotechnologies Institute (BIAM)

Summary

Automatically generated

Uranium is known to affect bone metabolism. Here, we present a protocol aimed at investigating the effect of natural uranium exposure on the viability, the differentiation, and the function of osteoclasts, the cells in charge of bone resorption.

Explore More Videos

OsteoclastogenesisUranium ExposureRAW 264.7 CellsCell CultureCell Seeding DensityUranium Salt SolutionRANKLOsteoclast DifferentiationOsteoclast FunctionEnvironmental ToxicityBone PhysiologyBone Metabolism

Read Article

Cite this Article

Gritsaenko, T., Pierrefite-Carle, V., Creff, G., Vidaud, C., Carle, G., Santucci-Darmanin, S. Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis. J. Vis. Exp. (131), e56499, doi:10.3791/56499 (2018).

Download .ris file

Copy

Share Video

.

Copy