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JoVE Journal Developmental Biology
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
 

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.

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Quantitative Whole-mount Immunofluorescence Analysis Cardiac Progenitor Populations Mouse Embryos Cardiac Progenitor Cell Localization Heart Organogenesis Volumetric Imagery Tissue Level Dynamics Automated Analysis Pipeline Reproducibility Embryogenesis Morphogenesis Organs Of Interest Pregnant Mouse Abdomen Abdominal Incision Cervix Uterus Mesometrium Embryos
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