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Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

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10:17 min

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January 31, 2016

DOI:

10.3791/53830-v

DOI:
10.3791/53830-v

•

10:17 min
January 31, 2016

•

7 Views
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1Department of Molecular Biosciences, The Wenner-Gren Institute (MBW),Stockholm University

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We describe a multi-target chromogenic whole-mount in situ hybridization (MC-WISH) procedure in intact Drosophila embryos allowing simultaneous and specific detection of three different mRNA distribution patterns by contrasting color precipitates.

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Multi-targetChromogenicWhole-mount In Situ HybridizationGene ExpressionDrosophila EmbryosMRNA LocalizationMulti-colorGene Regulatory InteractionsEmbryonic DevelopmentOrganogenesis

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Hauptmann, G., Söll, I., Krautz, R., Theopold, U. Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos. J. Vis. Exp. (107), e53830, doi:10.3791/53830 (2016).

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