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人脑脊液 Endopeptidomic 分析的样品制备

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

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人脑脊液 Endopeptidomic 分析的样品制备

8,808 Views

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10:23 min

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December 04, 2017

DOI:

10.3791/56244-v

DOI:

10.3791/56244-v

•

10:23 min

December 04, 2017

•

2 Views

Karl T. Hansson¹, Tobias Skillbäck^1,2, Elin Pernevik¹, Jessica Holmén-Larsson¹, Gunnar Brinkmalm¹, Kaj Blennow^1,2, Henrik Zetterberg^1,2,3, Johan Gobom^1,2

¹Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry,Sahlgrenska Academy at University of Gothenburg, ²Clinical Neurochemistry Laboratory,Sahlgrenska University Hospital, ³Department of Molecular Neuroscience,UCL Institute of Neurology

Summary

提出了一种人脑脊液内源性多肽的质谱分析方法。通过采用分子量切断过滤、色谱预分馏、质谱分析以及随后的多肽识别策略组合, 就可以将已知的脑脊液 peptidome 扩大近十倍以前的研究。

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