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JoVE Journal Neuroscience
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
 

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Article DOI: 10.3791/56244-v • 10:23 min • December 4th, 2017
December 4th, 2017 •
1, 1,2, 1, 1, 1, 1,2, 1,2,3, 1,2
1Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry, Sahlgrenska Academy at University of Gothenburg, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3Department of Molecular Neuroscience, UCL Institute of Neurology

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A method for mass spectrometric analysis of endogenous peptides in human cerebrospinal fluid (CSF) is presented. By employing molecular weight cut-off filtration, chromatographic pre-fractionation, mass spectrometric analysis and a subsequent combination of peptide identification strategies, it was possible to expand the known CSF peptidome nearly ten-fold compared to previous studies.

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Sample Preparation Endopeptidomic Analysis Human Cerebrospinal Fluid LC-MS Analysis Peptide Pre-fractionation Identified Peptides Low Abundance CSF Peptides Neurodegenerative Disorders Biomarkers Mass Spectrometry Clinical Studies Plasma Samples Tissue Samples Thawing CSF Buffering Agent Guanidinium Hydrochloride Protein Aggregates TCEP Incubation
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