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Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

8,796 Views

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10:23 min

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December 04, 2017

DOI:

10.3791/56244-v

DOI:

10.3791/56244-v

•

10:23 min

December 04, 2017

•

7 Views

Karl T. Hansson, Tobias Skillbäck², Elin Pernevik, Jessica Holmén-Larsson, Gunnar Brinkmalm, Kaj Blennow², Henrik Zetterberg^2,3, Johan Gobom²

¹Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry,Sahlgrenska Academy at University of Gothenburg, ²Clinical Neurochemistry Laboratory,Sahlgrenska University Hospital, ³Department of Molecular Neuroscience,UCL Institute of Neurology

Tags

Endopeptidomic Analysis Cerebrospinal Fluid Sample Preparation LC-MS Peptide Pre-fractionation Endogenous Peptides Biomarkers Neurodegenerative Disorders MWCO Filtration High-pH Reverse Phase HPLC Mass Spectrometry

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