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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

DOI:
10.3791/56234-v

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06:24 min

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December 15, 2017

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, , , ,
1IBG-1: Biotechnology,Forschungszentrum Jülich, 2Research Center Jülich,Bioeconomy Science Center (BioSC), 3Computational Systems Biotechnology (AVT.CSB),RWTH Aachen University, 4Institute for Biotechnology,RWTH Aachen University

Capítulos

  • 00:05Título
  • 01:05Seed Culture, Automated Media Preparation, and Start of Main Culture
  • 02:53Product Quantification and Preprocessing of Raw Data for Analysis
  • 04:22Results: Iterative Workflow with Final Statistical Evaluation of Results
  • 05:31Conclusion

Summary

Tadução automática

Tadução automática

This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.

Tags

Keywords: Heterologous Protein ProductionAutomated Microbioreactor TechnologyKringing Based Experimental DesignMicrobial Cultivation Media OptimizationBioprocess DevelopmentStrain PhenotypingDeep Well PlatesSeed CultureRobotic WorkflowMedia PreparationOptical DensityMain CulturesCultivation Microtiter PlatesBiolector DeviceStock Solutions DisposalLabware CleaningLiquid Handler Decontamination

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