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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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December 15, 2017
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This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.
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Heterologous Protein ProductionAutomated Microbioreactor TechnologyKringing Based Experimental DesignMicrobial Cultivation Media OptimizationBioprocess DevelopmentStrain PhenotypingDeep Well PlatesSeed CultureRobotic WorkflowMedia PreparationOptical DensityMain CulturesCultivation Microtiter PlatesBiolector DeviceStock Solutions DisposalLabware CleaningLiquid Handler Decontamination
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Hemmerich, J., Freier, L., Wiechert, W., von Lieres, E., Oldiges, M. Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology. J. Vis. Exp. (130), e56234, doi:10.3791/56234 (2017).
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