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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Chapters
- 00:05Title
- 01:05Seed Culture, Automated Media Preparation, and Start of Main Culture
- 02:53Product Quantification and Preprocessing of Raw Data for Analysis
- 04:22Results: Iterative Workflow with Final Statistical Evaluation of Results
- 05:31Conclusion
This manuscript describes a generic approach for tailor-made design of microbial cultivation media. This is enabled by an iterative workflow combining Kriging-based experimental design and microbioreactor technology for sufficient cultivation throughput, which is supported by lab robotics to increase reliability and speed in liquid handling media preparation.
Tags
Heterologous Protein ProductionAutomated Microbioreactor TechnologyKringing Based Experimental DesignMicrobial Cultivation Media OptimizationBioprocess DevelopmentStrain PhenotypingDeep Well PlatesSeed CultureRobotic WorkflowMedia PreparationOptical DensityMain CulturesCultivation Microtiter PlatesBiolector DeviceStock Solutions DisposalLabware CleaningLiquid Handler Decontamination
