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斑马鱼胚胎卵黄细胞中的 AFM 和 Microrheology
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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斑马鱼胚胎卵黄细胞中的 AFM 和 Microrheology

8,070 Views

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09:47 min

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November 29, 2017

DOI:

10.3791/56224-v

DOI:
10.3791/56224-v

•

09:47 min
November 29, 2017

•

95 Views
, , , , ,
1Instituto de Biología Molecular de Barcelona,Consejo Superior de Investigaciones Científicas, 2Institute for Bioengineering of Catalonia,Universitat de Barcelona and CIBER Enfermedades Respiratorias

Summary

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缺少用于测量材料属性和拉伸参数的工具在体内可防止在开发过程中验证它们的角色。我们使用原子力显微镜 (AFM) 和纳米粒子跟踪来量化外包期间完整的斑马鱼胚胎卵黄细胞的机械特性。这些方法是可靠的, 广泛适用于避免介入干预。

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Cite this Article

Marsal, M., Jorba, I., Rebollo, E., Luque, T., Navajas, D., Martín-Blanco, E. AFM and Microrheology in the Zebrafish Embryo Yolk Cell. J. Vis. Exp. (129), e56224, doi:10.3791/56224 (2017).

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