Journal
/
Bioquímica
/
氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
Please note that all translations are automatically generated. Click here for the English version.

氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布

DOI:
10.3791/56037-v

•

08:24 min

•

August 18, 2017

•

,
1Langone Medical Center,New York University

Capítulos

  • 00:05Título
  • 00:41Cell Harvesting
  • 01:35Nitrogen Cavitation
  • 03:31Separation of Cytosolic and Membrane Fractions
  • 05:22Results: Partitioning of Cellular Proteins in Membrane and Cytosolic Fractions
  • 07:07Conclusion

Summary

Tadução automática

Tadução automática

在这里我们目前用于洗涤剂免费培养的哺乳动物细胞基于氮空和随后的分离，胞浆和膜结合蛋白的超速离心同质化的协议。此方法是理想的监测分区之间可溶性的外周膜蛋白和膜组分。

Tags

Nitrogen CavitationDifferential CentrifugationPeripheral Membrane ProteinsCultured CellsSubcellular CompartmentalizationHEK293 CellsHomogenization BufferPressurizationProtease InhibitorCell DisruptionFractionationSoluble FractionMembrane Fraction

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em

巨囊泡封装微球通过一个水包油乳液的离心制备

生物素化细胞表面蛋白的纯化<emRigicephalus microplus</em>上皮细胞

线粒体钙在分离线粒体和培养细胞中的流入分析

用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法

细胞细胞接触物蛋白质-蛋白质相互作用的荧光波动光谱测定

高速离心条件下 u937 细胞的细胞分裂

实时测量核苷酸与完整功能性膜蛋白的结合

成熟水稻的横向切片（米 苜蓿L.）使用移液器吸头作为固定支架的扫描电子显微镜成像的内核

无细胞生产蛋白脂质体，用于功能分析和靶向膜蛋白的抗体开发

Read Article