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高脂膳食喂养和高通量三测定在果蝇中的含量

JoVE Journal
Genética

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JoVE Journal Genética

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

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高脂膳食喂养和高通量三测定在果蝇中的含量

10,808 Views

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08:28 min

•

September 13, 2017

DOI:

10.3791/56029-v

DOI:

10.3791/56029-v

•

08:28 min

September 13, 2017

•

4 Views

Soda Balla Diop¹, Ryan T. Birse¹, Rolf Bodmer¹

¹Development, Aging and Regeneration Program,Sanford Burnham Prebys Medical Discovery Institute

Summary

这是一个高脂肪饮食喂养协议, 以诱发肥胖在果蝇, 一个模型, 了解的基本分子机制牵连脂。它还提供了一种高通量三测定方法, 用于测量在各种饮食、环境、遗传或生理条件下的果蝇中的脂肪积累和潜在的其他 (昆虫) 模型。

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