Journal
/
Imunologia e Infecção
/
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

DOI:
10.3791/55993-v

•

09:22 min

•

October 13, 2017

•

, , , , , ,
1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Capítulos

  • 00:05Título
  • 00:42Elution of Tear Sample from Tear Flow Strip
  • 01:26Cytokine Profiling by Bead Based Multiplex Assay
  • 04:43Reading the Assay Plate and Data Analysis
  • 06:56Results: Tear Cytokine Profiles from Four Male Subjects
  • 08:54Conclusion

Summary

Tadução automática

Tadução automática

Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.

Tags

Tear Cytokine ProfileBead-based Multiplex AssaySchirmer StripOphthalmologyCytokinesOcular DiseasesAnalytesTear SampleAssay BufferCentrifugationCytokine ProfilingMultiplex AssayCytokine StandardsAssay WorksheetMagnetic Plate Washer

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to Plasmodium falciparum Parasites by a Flow Cytometry-Based Assay

Read Article