Journal
/
Neurociencias
/
A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay

A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay

DOI:
10.3791/55939-v

•

06:45 min

•

July 26, 2017

•

, ,
1Department of Biology,Queen’s University, 2Centre for Neuroscience,Queen’s University

Capítulos

  • 00:05Título
  • 00:42Preparation of Experimental Organisms
  • 01:15Preparation of Assay Plates
  • 02:31Chemotaxis Assay
  • 05:31Results: npr-9 Overexpression Nematodes Do Not Initiate Aversion to Copper
  • 06:21Conclusion

Summary

Traducción Automática

Traducción Automática

Here, we present a Caenorhabditis elegans-specific assay designed to evaluate changes in copper aversion behavior and the ability to locate a common food source, as the organism progresses from a well-fed to starved nutritional state.

Tags

Caenorhabditis ElegansCopper Aversion AssayNutritional StatusBehavioral NeuroscienceL4 Stage NematodesOP50 E. ColiCopper(II)sulfateFood PatchMidline Barrier

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Automated Quantification of Synaptic Fluorescence in C. elegans

A Caenorhabditis elegans Model System for Amylopathy Study

Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans

An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of Caenorhabditis elegans

Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Imaging Dendritic Spines in Caenorhabditis elegans

Read Article