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小容积回收-灌注-浸没式室系统中维持的急性海马切片的突触可塑性记录
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Neurociência
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JoVE Journal Neurociência
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
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小容积回收-灌注-浸没式室系统中维持的急性海马切片的突触可塑性记录

DOI:
10.3791/55936-v

•

09:51 min

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January 01, 2018

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, , ,
1The Institutes of Brain Science, the State Key Laboratory of Medical Neurobiology, the Collaborative Innovation Center for Brain Science,Fudan University

Capítulos

  • 00:05Título
  • 01:05Preparation of Acute Hippocampal Slices
  • 02:21Modifications of the Carbogenation for the aCSF Recycling of Small Reservoirs
  • 03:08Recording Synaptic Responses in a Submersion Slice Chamber
  • 07:28Results: Representative Recordings of Activity-dependent Potentiaation of Synaptic Transmission Using Outflow-carbogenation and Reservoir-carbogenation
  • 08:08Conclusion

Summary

Tadução automática

Tadução automática

本协议描述了在少量回收的缓冲液中的氧水平的稳定, 以及在淹没的急性海马切片中记录活动依赖性突触可塑性的方法学方面。

Tags

Synaptic PlasticityAcute Hippocampal SlicesRecycling-perfusion-submersion ChamberCarbogenationField Potential RecordingsNeuroplasticitySynaptic TransmissionMemory FormationOxygen StabilizationAcute Slice PreparationVibratomeHippocampal FormationRecycling SystemCarbogen Supply

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