Journal
/
Imunologia e Infecção
/
来自新世界Zika病毒感染性克隆的重组病毒的救援和表征
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone
Please note that all translations are automatically generated. Click here for the English version.

来自新世界Zika病毒感染性克隆的重组病毒的救援和表征

DOI:
10.3791/55857-v

•

07:35 min

•

June 07, 2017

•

, , , ,
1Department of Microbiology, Immunology, and Pathology,Colorado State University, 2Division of Vector-Borne Diseases,Centers for Disease Control and Prevention

Capítulos

  • 00:05Título
  • 00:40Preparation of In Vitro Transcribed RNA
  • 02:54Rescue of Infectious Virus by Electroporation
  • 05:56Results: In Vitro and In Vivo Characteristics of Clone-derived Virus
  • 07:02Conclusion

Summary

Tadução automática

Tadução automática

该方案描述了从双质粒感染性cDNA克隆中恢复感染性Zika病毒。

Tags

Zika VirusInfectious CloneRecombinant VirusFlavivirusPlasmid DigestionLigationIn Vitro TranscriptionVero CellsElectroporation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

质粒DNA重组流感病毒的一代

从现场收集到的蚊子传染病毒Vero细胞培养法检测

宣传和检测Maedi visna病毒传染性表达绿色荧光蛋白的分子克隆

从救援的cDNA重组新城疫病毒

中性粒细胞分离和分析，以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用

兹卡病毒感染细胞培养系统和<em>体外</em>干扰素的预防作用

Zika 病毒特定诊断表位发现

基于流式细胞仪的 Zika 病毒疗养血清测定登革热病毒的体外抗体依赖性

快速、安全、简便的人工床边核酸提取法检测全血标本中的病毒

原代人细胞中寨卡病毒抗体依赖性增强的定量研究

Read Article