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应用酵母雌激素筛检优选本科教学实验室的个人护理产品中的雌激素配体
JoVE Journal
Biologia
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JoVE Journal Biologia
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
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应用酵母雌激素筛检优选本科教学实验室的个人护理产品中的雌激素配体

12,447 Views

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14:13 min

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January 01, 2018

DOI:

10.3791/55754-v

DOI:
10.3791/55754-v

•

14:13 min
January 01, 2018

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8 Views
, , , , ,
1Department of Biology,University of the South, 2School of Biological Sciences,Louisiana Tech University, 3School of Medicine,Louisiana State University Health Sciences Center, 4Department of Biology,Furman University, 5Department of Computer Science,Louisiana Tech University, 6Clemson University

Summary

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本文介绍了一个优化酵母雌激素屏幕的量化配体的个人护理产品 (杆), 结合雌激素受体α (ERα) 和/或β (er)。该方法采用了两种色度基质选择, 一个六天的冷冻孵化, 用于本科课程, 以及数据分析的统计工具。

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Cite this Article

Edwards, T. M., Morgan, H. E., Balasca, C., Chalasani, N. K., Yam, L., Roark, A. M. Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory. J. Vis. Exp. (131), e55754, doi:10.3791/55754 (2018).

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