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Biology

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Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
 

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Article DOI: 10.3791/55754-v • 14:13 min • January 1st, 2018
January 1st, 2018 •
1,2, 2,3, 4, 5, 4,6, 4
1Department of Biology, University of the South, 2School of Biological Sciences, Louisiana Tech University, 3School of Medicine, Louisiana State University Health Sciences Center, 4Department of Biology, Furman University, 5Department of Computer Science, Louisiana Tech University, 6Clemson University

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This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.

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Keywords: Yeast Estrogen Screen Personal Care Products Environmental Estrogens Endocrine Disruption Ecophysiology In Vitro Undergraduate Teaching Reagent Preparation Sample Extraction Centrifugation Ethanol Evaporation
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