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Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory
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生物学
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JoVE Journal 生物学
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

12,448 Views

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14:13 min

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January 01, 2018

DOI:

10.3791/55754-v

DOI:
10.3791/55754-v

•

14:13 min
January 01, 2018

•

8 Views
, , , , ,
1生物学科,University of the South, 2School of Biological Sciences,Louisiana Tech University, 3School of Medicine,Louisiana State University Health Sciences Center, 4生物学科,Furman University, 5Department of Computer Science,Louisiana Tech University, 6Clemson University

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This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.

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Edwards, T. M., Morgan, H. E., Balasca, C., Chalasani, N. K., Yam, L., Roark, A. M. Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory. J. Vis. Exp. (131), e55754, doi:10.3791/55754 (2018).

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