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Personalized Needles for Microinjections in the Rodent Brain
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Neurociência
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JoVE Journal Neurociência
Personalized Needles for Microinjections in the Rodent Brain

Personalized Needles for Microinjections in the Rodent Brain

DOI:
10.3791/55751-v

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10:06 min

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January 24, 2018

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, , , , , , , , ,
1Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences,University of Ferrara, 2Laboratory for the Technologies for Advanced Therapies (LTTA),University of Ferrara

Capítulos

  • 00:05Título
  • 00:56Preparation of Quartz Needles
  • 03:18Microinjection
  • 08:03Results: Coronal Sections Showing Less Damage Using the Quartz Needle
  • 08:56Conclusion

Summary

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Tadução automática

We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.

Tags

Personalized Quartz NeedlesMicroinjectionRodent BrainNeurociênciaNeurodegenerative DiseasesPsychiatric DiseasesGene TherapyCell TherapyCost-effectiveReusableLaser PullerMicro DissectorVoid PumpFlow Rate Calibration

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