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Personalized Needles for Microinjections in the Rodent Brain
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神经科学
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JoVE 杂志 神经科学
Personalized Needles for Microinjections in the Rodent Brain

Personalized Needles for Microinjections in the Rodent Brain

DOI:
10.3791/55751-v

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10:06 min

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January 24, 2018

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, , , , , , , , ,
1Department of Medical Sciences, Section of Pharmacology, and National Institute of Neurosciences,University of Ferrara, 2Laboratory for the Technologies for Advanced Therapies (LTTA),University of Ferrara

Chapters

  • 00:05Title
  • 00:56Preparation of Quartz Needles
  • 03:18Microinjection
  • 08:03Results: Coronal Sections Showing Less Damage Using the Quartz Needle
  • 08:56Conclusion

Summary

自动翻译

自动翻译

We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.

Tags

Keywords: Personalized Quartz NeedlesMicroinjectionRodent Brain神经科学Neurodegenerative DiseasesPsychiatric DiseasesGene TherapyCell TherapyCost-effectiveReusableLaser PullerMicro DissectorVoid PumpFlow Rate Calibration

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