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Zuivering van Biotinylated Cell Surface Proteins uit<em> Rhipicephalus microplus</em> Epitheliale darmcellen
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JoVE Journal Bioquímica
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
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Zuivering van Biotinylated Cell Surface Proteins uit<em> Rhipicephalus microplus</em> Epitheliale darmcellen

9,259 Views

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12:03 min

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July 23, 2017

DOI:

10.3791/55747-v

DOI:
10.3791/55747-v

•

12:03 min
July 23, 2017

•

3 Views
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1Queensland Alliance for Agriculture & Food Innovation,The University of Queensland, 2Centre for Comparative Genomics,Murdoch University

Summary

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Een gemodificeerde dichtheidscentrifugatiegradient gebaseerde methodologie werd gebruikt om epitheelcellen van Rhipicephalus microplus gutweefsel te isoleren. Oppervlakte gebonden eiwitten werden biotinyleerd en gezuiverd door streptavidine magnetische kralen voor gebruik in downstream toepassingen.

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Cite this Article

Karbanowicz, T. P., Lew-Tabor, A., Rodriguez Valle, M. Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells. J. Vis. Exp. (125), e55747, doi:10.3791/55747 (2017).

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