Dergi
/
Gelişim Biyolojisi
/
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

DOI:
10.3791/55515-v

•

07:18 min

•

May 12, 2017

•

, ,
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, 2Department of Cancer Biology,Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology,Harvard Tıp Fakültesi

Bölümler

  • 00:05Başlık
  • 00:47mESC Culture Conditions and Procedures for Screen Preparation
  • 03:09Kinase Inhibitor Screening Analysis
  • 04:56Results: Identification of Kinase Inhibitors that Modulate mESC Pluripotency
  • 06:13Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present a quantitative and scalable protocol to perform targeted small molecule screens for kinase regulators of the naïve-primed pluripotent transition.

Etiketler

Embryonic Stem CellPluripotencyKinase Inhibitor ScreeningHigh-throughputNovel Signaling PathwaysMouse ES CellsGelatin CoatingCell CultureTrypsinizationCell Counting96-well PlateKinase Inhibitor Treatment

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

A Direct and Simple Method to Assess Drosophila melanogaster‘s Viability from Embryo to Adult

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Read Article