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受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
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受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

24,783 Views

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07:46 min

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April 03, 2017

DOI:

10.3791/55445-v

DOI:
10.3791/55445-v

•

07:46 min
April 03, 2017

•

8 Views
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1Department of Veterinary and Biomedical Sciences,The Pennsylvania State University, 2Microscopy and Cytometry Facility, The Huck Institutes of Life Sciences,Pennsylvania State University

Summary

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该协议允许研究员分离和表征组织驻留巨噬细胞在各种标志发炎组织提取从饮食诱发的代谢紊乱模型。

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Allen, J. N., Dey, A., Nissly, R., Fraser, J., Yu, S., Balandaram, G., Peters, J. M., Hankey-Giblin, P. A. Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation. J. Vis. Exp. (122), e55445, doi:10.3791/55445 (2017).

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