Journal
/
Neurociência
/
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models

DOI:
10.3791/55418-v

•

10:46 min

•

May 03, 2017

•

, , , , ,
1Department of Psychology,West Virginia University, 2Department of Drug Discovery & Development,Auburn University, 3Department of Neuroscience,University of Kentucky Medical Center

Capítulos

  • 00:05Título
  • 00:50Coating the MEA
  • 01:40Electroplating the Electrode
  • 02:46Preparing the Solutions Needed for Calibration
  • 04:48Assemble the Micropipette
  • 05:46General Animal Surgery for MEA Recordings
  • 09:01Results: Extracellular Tonic and Potassium Chloride-evoked Release of Glutamate in Hippocampus
  • 10:18Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe the setup, software navigation, and data analysis for a spatially and temporally precise method of measuring tonic and phasic extracellular glutamate changes in vivo using enzyme-linked microelectrode arrays (MEA).

Tags

Enzyme-based BiosensorsTonic And Phasic GlutamateAlzheimer’s Mouse ModelsMicroElectrode ArraysNeurotransmitter LevelsNeurotransmitter Release And ClearanceSpatially And Temporally PreciseGlutamate OxidasePBSElectroplatingCalibrationHeating PadPBS Solution

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Mouse Models of Periventricular Leukomalacia

Hyponeophagia: A Measure of Anxiety in the Mouse

Detection of Neuritic Plaques in Alzheimer’s Disease Mouse Model

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Read Article