Two image analysis algorithms, "Drosophila NMJ Morphometrics" and "Drosophila NMJ Bouton Morphometrics" were created, to automatically quantify nine morphological features of the Drosophila neuromuscular junction (NMJ).
Castells-Nobau, A., Nijhof, B., Eidhof, I., Wolf, L., Scheffer-de Gooyert, J. M., Monedero, I., Torroja, L., van der Laak, J. A., Schenck, A. Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology. J. Vis. Exp. (123), e55395, doi:10.3791/55395 (2017).