Two Algorithms for High-throughput and Multi-parametric Quantification of <em>Drosophila</em> Neuromuscular Junction Morphology

Anna Castells-Nobau; Bonnie Nijhof; Ilse Eidhof; Louis Wolf; Jolanda M. Scheffer-de Gooyert; Ignacio Monedero; Laura Torroja; Jeroen A.W.M. van der Laak; Annette Schenck

doi:10.3791/55395

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Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology

Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology

DOI:

10.3791/55395-v

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12:29 min

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May 03, 2017

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Anna Castells-Nobau*¹, Bonnie Nijhof*¹, Ilse Eidhof, Louis Wolf, Jolanda M. Scheffer-de Gooyert, Ignacio Monedero⁴, Laura Torroja, Jeroen A.W.M. van der Laak⁵, Annette Schenck*¹

¹Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour,Radboud University Medical Center, ²Microscopical Imaging Centre (MIC),Radboud University Medical Center, ³Department of Biology,Universidad Autónoma de Madrid, ⁴Department of Clinical and Experimental Medicine,Linköping University, ⁵Department of Pathology,Radboud University Medical Center

Chapitres

00:05Titre
01:03Creating NMJ Images with Defined Features
04:50Macro Settings: Rolling Ball Radius Value and Auto-threshold
07:26Macro Settings: Maxima Noise Tolerance Value, and Brp-puncta Thresholds
10:16Results: Validating the Marco by Recapitulating Known NMJ Defects
11:38Conclusion

Summary

Traduction automatique

Two image analysis algorithms, "Drosophila NMJ Morphometrics" and "Drosophila NMJ Bouton Morphometrics" were created, to automatically quantify nine morphological features of the Drosophila neuromuscular junction (NMJ).

Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology

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Chapitres

Summary

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