Journal
/
Biologia do Desenvolvimento
/
Using Primary Neurosphere Cultures to Study Primary Cilia
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
Using Primary Neurosphere Cultures to Study Primary Cilia

Using Primary Neurosphere Cultures to Study Primary Cilia

DOI:
10.3791/55315-v

•

08:14 min

•

April 14, 2017

•

, , ,
1Department of Cell Biology,University of Texas Southwestern Medical Center

Capítulos

  • 00:05Título
  • 01:13Isolation of Neurospheres from the Adult Mouse Brain
  • 02:43Embedding and Sectioning of Neurospheres for immunostaining
  • 04:10Starvation and Analysis of Cilia
  • 06:01Results: Starvation Induces Ciliogenesis in Dissociated, Adherent Neutral Stem/Progenitor Cells
  • 07:19Conclusion

Summary

Tadução automática

Tadução automática

The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.

Tags

Keywords: Primary Neurosphere CulturesPrimary CiliaNeural Stem CellsNeural ProgenitorsNeuronsLateral VentricleSubventricular ZoneTrypsin-EDTABasal MediumNeurospheresImmunostainingParaformaldehydeSucrose

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/55315?article_type=v

Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
check_url/pt/55315?article_type=v

Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
check_url/pt/55315?article_type=v

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
check_url/pt/55315?article_type=v

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
check_url/pt/55315?article_type=v

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
check_url/pt/55315?article_type=v

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
check_url/pt/55315?article_type=v

Generation and Culturing of Primary Human Keratinocytes from Adult Skin
check_url/pt/55315?article_type=v

Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
check_url/pt/55315?article_type=v

Induction and Validation of Cellular Senescence in Primary Human Cells
check_url/pt/55315?article_type=v

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Read Article